空气污染与动物水平研究有关科研的主要方法
- 发布人：
- 发布日期：2017-04-21 11:34:18
（一）在体研究：
整体（全身）暴露：被普遍认为是最接近模拟人的现实暴露的方式。对PM2.5暴露来说，目前主要有浓缩暴露系统和非浓缩（现实浓度）暴露系统。代表性参考文献如下：
浓缩系统：Xu X, et al. Long-term exposure to ambient fine particulate pollution induces insulin resistance and mitochondrial alteration in adipose tissue. Toxicol Sci. 2011;124(1):88-98
非浓缩系统：Xu X, et al. Association of systemic inflammation with marked changes in particulate air pollution in Beijing in 2008. Toxicol Lett. 2012;212(2):147-56
“开放”(Open top)系统：Mauad T, et al. Chronic exposure to ambient levels of urban particles affects mouse lung development. Am J Respir Crit Care Med. 2008;178(7):721-8.
罩棚(shed)系统：Somers CM, et al. Reduction of particulate air pollution lowers the risk of heritable mutations in mice. Science. 2004;304(5673):1008-10
经鼻暴露：多为将颗粒物溶入细胞培养液中经鼻腔暴露，比较“机械”但仍属于“经气管”的渠道。代表性参考文献如下：
Arantes-Costa FM, et al. Effects of residual oil fly ash (ROFA) in mice with chronic allergic pulmonary inflammation. Toxicol Pathol. 2008;36(5):680-6
